It is known that metabolic syndrome (MS) prolongs the healing time of the wound surface of various genesis. We hypothesized that this may be related to changes in the biophysical properties of the skin. The aim of the work was to investigate the biophysical properties of skin (content of moisture, collagen, fused gelatin in the skin, and skin thermal fusion temperature) in rats with MS.

Materials and methods. Research was conducted on 40 white non-linear rats (20 – males, 20 – females). After birth, males and females were divided into 2 groups. The first group served as a control for the second groups, in which MS was simulated by administering monosodium glutamate at a dose of 4.0 mg/kg on the 2nd, 4th, 6th, 8th, and 10th postnatal days. At the age of 4 months, the animals were decapitated under ether anesthesia. Biochemical studies were conducted to confirm the development of MS. The percentage of moisture, collagen content, destructive changes in collagen fibers and resistance of skin collagen to thermal factors were determined in native skin.

The results. 4-month-old rats injected with monosodium glutamate in the early neonatal period developed MS as evidenced by abdominal obesity, insulin resistance, and dyslipidemia. In comparison with animals of the control group, in the skin of male and female rats with MS, the moisture content decreased, and the collagen content did not change. At the same time, the percentage of melted gelatin doubled, which indicates the depth of destructive changes in collagen fibers. Thermal welding of skin samples of male and female rats with signs of MS occurred at rather low temperatures compared to rats of the control group.

Conclusion. Changes in the biophysical properties of the skin, which are registered in rats with MS, may be the reason for the later closure of the wound surface.

Article received: 01.02.2024

Keywords: metabolic syndrome, skin moisture, collagen, gelatin, hydrothermal skin welding

Full text: PDF (Ukr)

1. Favaro L, Masini F, Maffei ML, Botti G. [Syndrome X]. Recenti Prog Med. 1989;80:281-285.
2. Rochlani Y, Pothineni NV, Kovelamudi S, Mehta JL. Metabolic syndrome: pathophysiology,management, and modulation by natural compounds. Ther Adv Cardiovasc Dis. 2017;11:215-25.doi.org/10.1177/1753944717711379
3. Samson SL, Garber AJ. Metabolic syndrome. Endocrinol Metab Clin North Am. 2014;43:1-23.doi.org/10.1016/j.ecl.2013.09.009
4. Karadag AS, Lavery MJ. Skin and the metabolic syndrome. Clin Dermatol. 2018;36:1-2.doi.org/10.1016/j.clindermatol.2017.09.001
5. Karadag AS, You Y, Danarti R, Al-Khuzaei S, Chen W. Acanthosis nigricans and the metabolic syndrome. Clin Dermatol. 2018;36:48-53.doi.org/10.1016/j.clindermatol.2017.09.008
6. Gui XY, Yu XL, Jin HZ, Zuo YG, Wu C. Prevalence of metabolic syndrome in Chinese psoriasis patients: a hospital-based cross-sectional study. J Diabetes Investig. 2018;9:39-43.doi.org/10.1111/jdi.12663
7. Singh S, Young P, Armstrong AW. Relationship between psoriasis and metabolic syndrome: a systematic review. G Ital Dermatol Venereol. 2016;151:663-77.
8. Adibi N, Robati RM. Skin and metabolic syndrome: A review of the possible associations. J Res Med Scie. 2021 Feb;26:16.doi.org/10.4103/jrms.JRMS_585_20
9. Hu Y, Zhu Y, Lian N, Chen M, Bartke A, Yuan R. Metabolic syndrome and Skin Diseases. Frontiers in Endocrynology. November 2019;10:788.doi.org/10.3389/fendo.2019.00788
10. Nagpal M, De D, Handa S, Pal A, Sachdeva N. Insulin resistance and metabolic syndrome in young men with acne. JAMA Dermatol. 2016;152:399-404.doi.org/10.1001/jamadermatol.2015.4499
11. Rodrigues M, Kosaric N, Bonham CA, Gurtner GC. Wound Healing: A Cellular Perspective. Physiol Rev. 2019;99:665-706.doi.org/10.1152/physrev.00067.2017
12. Wilkinson HN, Hardman MJ. Wound healing: cellular mechanisms and pathological outcomes. Open Biol. 2020.doi.org/10.1098/rsob.200223
13. Patel S, Srivastava S, Singh MR, Singh D. Mechanistic insight into diabetic wounds:Pathogenesis, molecular targets and treatment strategies to pace wound healing. Biomed Pharmacother. 2019;112:108615.doi.org/10.1016/j.biopha.2019.108615
14. Strollo F, Gentile S, Pippcelli AMV, Mambro A, Monici M, Magni P. Space Flight-Promoted Insulin Resistance as a Possible Disruptor of Wound Healing. Frontiers in Bioengineering and Biotechnology. May 2022;10: 868999.doi.org/10.3389/fbioe.2022.868999
15. Sanabria ER, Pereira MF, Dolnikoff MS, Andrade IS, Ferreira AT, Cavalheiro EA, Fernandes MJ. Deficit in hippocampal long-term potentiation in monosodium glutamate-treated rats. Brain Res Bull. 2002;59:47-51.doi.org/10.1016/S0361-9230(02)00837-7
16. Vogeser M, Konig D, Frey I, Predel HG, Parhofer KG, Berg A. Fasting serum insulin and the homeostasis model of insulin resistance (HOMA-IR) in the monitoring of lifestyle interventions in obese persons. Clin Biochem. 2007;40:964-968.doi.org/10.1016/j.clinbiochem.2007.05.009
17. Bernardis LL, Patterson BD. Correlation between 'Lee index' and carcass fat content in weanling and adult female rats with hypothalamic lesions. J Endocrinol. 1968;40:527-528.doi.org/10.1677/joe.0.0400527
18. Верещака ВВ. Етіологія та патогенез старечої в'ялості шкіри лиця і механізми формування його структурних змін у сучасної людини європеоїдного типу. К. : Наук. думка, 2008. 481 с.
19. Хімічний аналіз і фізико-механічні випробування шкіри та хутра : методичні вказівки до лабораторних робот з дисципліни «Аналітичний контроль у шкіряно-хутровому виробництві» / Київ. нац. ун-т технологій та дизайну ; упоряд. : О. А. Охмат, В. П. Плаван, О. Р. Мокроусова, А. Г. Данилкович. К. : КНУТД, 2006. 64 с.
20. N.R. Hrytsevych, N.S. Nikitina, L.I. Stepanova , O.M. Savchuk , V.V. Vereshchaka.Content of growth factors and hypoxia-inducible factor 1α in the wound bed of the skin of rats with metabolic syndrome. Fiziol. Zh. 2023;69(6):69-76.doi.org/10.15407/fz69.06.069
21. Savcheniuk O, Kobyliak N, Kondro M, Virchenko O, Falalyeyeva T, Beregova T. Short-term periodic consumption of multiprobiotic from childhood improves insulin sensitivity, prevents development of non-alcoholic fatty liver disease and adiposity in adult rats with glutamate-induced obesity. BMC Complement. Altern. Med. 2014;14:247.doi.org/10.1186/1472-6882-14-247
22. Laaksonen DE, Nuutinen J, Lahtinen T, Rissanen A, Niskanen LK. Changes in abdominal subcutaneous fat water content wirh rapid weight loss and long-term weght maintenance in abdominally obese men and women. Int J Obes Relat Metab Disord. 2003 Jun;27(6):677-683.doi.org/10.1038/sj.ijo.0802296
23. Black MM, Bottoms E, Shuster S. Skin collagen and thickness in simple obesity. Br Med J. 1971;4:149-150.doi.org/10.1136/bmj.4.5780.149
24. 24. Akase T, Nagase T, Huang L, Ibuki A, Minematsu T, Nakagami G, Ohta Y, Shimada T, Aburada M, Sugama J, Sanada H. Aging-like skin changes induced by ultraviolet irradiation in an animal model of metabolic syndrome. Biol Res Nurs. 2012 Apr;14(2):180-187.doi.org/10.1177/1099800411401013