In order to study changes in the activity of secretory serum enzymes in patients with generalized periodontitis (GP) as a result of comprehensive treatment, there were examined 36 healthy persons and 132 patients with GP with chronic and acute course of the initial (45), the I st (45) and the II nd (42) degree of the disease, somatically healthy, aged 19-45 years. The activity of cholinesterase (CE) and ceruloplasmin (CP) was studied before, immediately after, after 6 and 12 months. There was a decrease in the activity of CE in GP of the initial degree, especially in the case of exacerbation (p 1 <0.05), and after treatment it significantly increased in all patients (p 2 <0.05–0.005) and became higher than normal. Instead, the activity of CP was increased, and due to the therapy it significantly decreased for six months and was close to normal. In the I st degree of the disease, the activity of CE was even lower, and the CP – was much higher. The normalization of activity was achieved by the treatment, especially immediately after it and after 6 months. The decrease in CE and increase in CP indices in GP of the II degree was the largest (p 1 <0.01–≤0.001). The treatment contributed to a significant increase in the activity of CE in the chronic course for the whole year, in the acute course – for six months, as well as a significant reduction in CP in the chronic course for six months, and in the acute course – for a year. The difference with the healthy people after therapy in all patients was insignificant. Strong reliable inverse correlations were found between the activity of CE and CP at all stages of the study. Therefore, significantly altered activity of CE and CP with the use of spirulina is successfully regulated, especially during the first six months, with the achievement of the norm for GP of the initial and I st degree.

Recieved: 15.02.2021

Keywords: generalized periodontitis, blood serum, secretory enzymes, complex treatment

Full text: PDF (Ukr)

1. 1. Volf GF, Rateytskhak EM, Rateytskhak K. Periodontology. M.: MEDpress-inform; 2008. 548 p.
2. 2. Taba MJr, Kinney J, Kim AS. Diagnostic biomarkers for oral and periodontal diseases . Dent. Clin. North. Am. 2005;49(3):551-71.
3. 3. Kuzenko YeI, Romaniuk AM. Inflammatory periodontal diseases: pathogenesis and morphogenesis: monograph. 2016. 137 p.
4. 4. Demkovych AYe, Bondarenko YuI. Major pathogenetic approaches to the prevention and treatment of inflammatory periodontal diseases. Bulletin of Scientific Research. 2015; 1:4-9.
5. 5. Pavlotska LF. Biological chemistry. Sumy: University book; 2009. 379 p.
6. 6. Sklyarova OYa, editor. Biochemical composition of body fluids and their clinical-diagnostic value. 2004. 192 p.
7. 7. Borysenko AV. Influence of periodontal diseases on the general condition of the organism. Public health. 2013;1:32-7).
8. 8. Chorna IV, Vysotsky IYu. Clinical enzymology. Enzymodiagnostics: textbook. Sumy: Sumy State University; 2013. 242 p.
9. 9. Borysenko AV. Biochemical substantiation of complex treatment of generalized periodontitis. Modern medical technologies. 2009;2:69-73.
10. 10. Melnychuk HM, Rozhko MM, Zaverbna LV. Gingivitis, periodontitis, periodontosis: peculiarities of treatment: textbook. 5th ed. 2011. 328 p.
11. 11. Babenko HO. Biosphere, anthropogenesis and health. Ivano-Frankivsk; 1999. 204 p.
12. 12. Luchak MV. Markers of early stages of damage to the hepatobiliary system in children living in regions with different types of environmental pollution [dissertation]. Lviv; 2016. 176 p.
13. 13. Kashivska RS. The state of periodontium in patients with generalized periodontitis in diseases of hepatobiliary system and the rationale for medicinal correction of identified disorders [dissertation]. Ivano-Frankivsk; 2016. 20 p.
14. 14. Apatzidou DA, Iskas A, Konstantinidis A, Alghamdi AM, Tumelty M, Lappin DF, Nile CJ. Clinical association between acetylcholine levels and cholinesterase activity in saliva and gingival crevicular fluid and periodontal diseases. J Clin Periodontol. 2018;45(10):1173-83.
15. 15. Lukinykh LM, Livshits YuN. Apical periodontitis. 2004. 86 p.
16. 16. Harshavardhana B, Rath SK, Mukherjee M. Evaluation of ceruloplasmin in aggressive and chronic periodontitis patients. J Indian Soc Periodontol. 2013;17(3):333-7.
17. 17. Polepalle T, Moogala S, Boggarapu S, Pesala DS, Palagi FB. Acute Phase Proteins and Their Role in Periodontitis: A Review J. Clin Diagn Res. 2015; 9(11):1-5.
18. 18. Shrinidhi Maji Shankar , KC Chethana, G Kavyashree , BG Soumya, Kala Bushan, TP Shiva Kumar. Assessment of Serum Ceruloplasmin Levels in Gingivitis, Chronic and Aggressive Periodontitis atients- A Clinico-Biochemical Studyv. J. of Clinical and Diagnostic Research. 2018;12(1):6-9.
19. 19. Anfilova MR, Myroniuk TM, Liashenko NI, Bodnar SA, Trunina TI. Spirulina and Silix in the complex treatment of patients with psoriasis and atopic dermatitis. Dermatology. Cosmetology. Sexopathology. 2006;1-2(9):170-4.
20. 20. Jaideep Mahendra, Little Mahendra, Jananni Muthu, Libby John, Georgios E Romanos. Clinical effects of subgingivally delivered spirulina gel in chronic periodontitis cases: a placebo controlled clinical trial. J Clin Diagn Res. 2013;7(10):2330-3.
21. 21. Radhika Maniyar, Umashankar GK. Effectiveness of spirulina mouthwash on the reduction of dental plaque and gingivitis: a clinical study. Int J. Pharm Pharm Sci. 2017;9(7):136-9.