Introduction. In this experimental research the influence of a HAES-LX-5% colloid-hyperosmolar solution and the reference preparation of a protein-salt solution Lactoprotein with sorbitol (LPS) and the control solution of 0.9% NaCl was studied on the functional state of the lungs according to the ultrastructural indices of rats’ lungs on cellular and subcellular levels. Materials and methods. The solutions were used in the dose of 10 ml/kg intravenous per day during 168 hours. The stereometric analysis of the lung tissue showed that in rats that were injected with solutions of 0.9% NaCl, HAES-LX-5% and LPS, there were no significant differences in the relative volume of damaged lung areas as compared to the ones of intact animals. During the experiment, this indicator varied from 0.071 to 0.111 cm3/cm3 (p > 0.05). From the first to the thirtieth day of the experiment the relative volume of the damaged areas of the lungs was significantly less (p < 0.001) in all groups of the experimental animals. Results. The changes in the morphological structure of the lungs of animals that were injected with infusion solutions were studied on the 7th, 14th and 30th days from the start of the experiment. The results of the study assessing the ultrastructure of rats' lungs, the bronchial, vascular and alveoli conducting systems suggest that the administration of HAES-LX-5% solution to animals does not cause pathological changes in the lungs ultrastructure: in the bronchial and vascular conducting systems, alveoli, air-blood barrier, alveolarocytes of II type and unlike the reference preparation, does not increase the adhesion of leukocytes to endotheliocytes and does not increase the number of alveolar macrophages, confirming its harmlessness and the absence of potential allergenic properties. Conclusions. The study confirmed the safety of these three solutions, the absence of their toxic effects on the structures of the rats’ lungs during the entire observation period (from the first day to the thirtieth one). The obtained results can be added to the pharmacological characteristics of the colloidal hyperosmolar preparation of HAES-LX-5% and the protein-salt hyperosmolar solution of LPS.

Recieved: 24.04.2019

Keywords: infusion therapy, colloid-hyperosmolar solutions, hydroxyethyl starch, Lactoprotein with sorbitol, safety, ultrastructure

Full text: PDF (Eng)

1. 1. Nagaichuk VI. Modern approaches to helping patients with burns. The Art of Treatment. 2010;5:24-9.
2. 2. Xiu F, Jeschke MG. Perturbed mononuclear phagocyte system in severely burned and septic patients. Shock. 2013;40(2):81-8. doi.org/10.1097/SHK.0b013e318299f774
3. 3. Klimenko MO, Netyukhaylo LG. Pathobiochemistry of metabolism. Poltava; 2012. 137.
4. 4. Kozinets GP, Cygankov VP, Kovalenko OM. Modern infusion therapy in burn shock. Ukrainian Journal of Hematology and Transfusiology. 2012;4(15):39-42.
5. 5. Shlapak IP, Netjazhenko VZ, Galushko OA. Infusion therapy in the practice of a doctor of internal medicine. K.: Logos; 2013. 307.
6. 6. Semenenko AI, Kondratsky BU, Yakovleva PA. Evaluation of the effect of a new colloid-hyperosmolar preparation on the central and hepatic hemodynamics in the conditions of experimental burn shock. Ukrainian Journal of Hematology and Transfusiology. 2010;2:31-5.
7. 7. Semenenko OM, Yakovleva NGO, Semenenko SI. Comparative evaluation of the therapeutic effect of solutions of NAES-LX-5% and Lactoprotein with sorbitol in conditions of burn disease according to the dynamics of indicators of bioenergetic processes in the kidneys. Clinical and Experimental Pathology. 2015;XIV,2(52):180-4.
8. 8. Makarova OI, Tchaikovsky AB. Features of ultrastructural changes in the respiratory part of the lungs of rats in the long-term period after thermal injury under conditions of its correction with colloid-hyperosmolar infusion solutions HAES-LX 5%. The World of Medicine and Biology. 2014,4(46):115-20.
9. 9. The complex colloid hyperosmolar infusion medication. Kondratskyi BO, Novak VL, Kondratskyi YB. Request N a 200908880; appl. 25.08.2009; publish. 10.03.2011, Bull N 5.
10. 10. Molchanov IW, Goldina OA, Gorbachevsky SE. Solutions of gilroksietilirovannogo starch - modern and effective plasma-substituting means of infusion therapy: monographic review. Moscow; 2003. 120.
11. 11. Baron JF. A new Hydroxyethyl Starch: HES 130/0.4, Voluven. Tranfusion Alternatives in Transfusion Medicine. 2009;2(2):13-21. doi.org/10.1111/j.1778-428X.2000.tb00028.x
12. 12. Ligen L, Hongming Y, Feng L, Chuanan S, Daifeng H, Xiaoye T. Morphologic changes and prognosis of the respiratory tract epithelium in inhalation injury and their relationship with clinical manifestations. Surgery. 2012;151(2):206-12. doi.org/10.1016/j.surg.2011.07.027
13. 13. Aubron C, Bellomo R. Infusion of hydroxyethyl starch-containing fluids. Minerva Anestesiol. 2013;79(9):1088-92.
14. 14. Malyshev IYu, Lyamina SV, Shimshelashvili ShL, Wasserman EN. Functions of alveolar macrophages and surfactant protein D in lung disease. Pulmonology. 2011;3:101-7). doi.org/10.18093/0869-0189-2011-0-3-101-107
15. 15. Preclinical studies of medications: methodical recommendations [red. OV Stefanov]. K.: Avicena; 2001. 528.
16. 16. Regas FC, Ehrlich HP. Elucidating the vascular response to burns with a new ret model. J. Trauma. 1992;32(5):557-63. doi.org/10.1097/00005373-199205000-00004
17. 17. Patent for invention 32467 Ukraine, IПК А 61 К 9/08, А 61 К 47/36. Plasma-like solution «Lactoprotein with sorbitol». Myndiuk MV, Kachorovskyi BV, Novak VL, Vynarchyk MJ, Kondratskyi BO, Orlyk VV. Request N a 200908880; appl. 25.08.2009; publish. 10.03.2011, Bull. N 5
18. 18. Volkova OМ, Yeletsky UK. Basics of histology with histological technique. M.: Medicine; 1982. 304.