The article analyzes the dynamics of lipid peroxidation (LPO) and activity of antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase) in erythrocytes of albino rats in experimental diabetes mellitus type 2 induced by five-time administration of streptozotocin (40 mg / kg body weight) and use of plant drugs containing quercetin flavonoids (50 mg / kg body weight daily for 30 days) as a correction factor. Diabetic condition in animals was monitored by way of determining the concentration of glucose in blood plasma and glycosylated hemoglobin level. In the course of investigations it had been established that administration of streptozotocin causes a twofold increase in glucose concentration in blood plasma of rats in the group D1 and glycated hemoglobin (HbA1c) – by 71.6 % at the 90th day of the experiment.

Changes in the identified indicator constants of diabetic condition give evidence of developing diabetes mellitus in experimental animals resulting from the cytotoxic effect of streptozotocin on pancreatic β-cells. Findings of the investigation of glucose concentration in plasma and HbA1c in blood of rats in the group D2 treated with quercetin on experimental streptozotocin diabetes background suggest that the indicated figures remain higher as compared to the control, but significantly decrease in comparison with the figures of animals with simulated streptozotocin diabetes. It has been established that the development of streptozotocin-induced diabetes is accompanied by accumulation of lipid peroxidation products (malondialdehyde), inhibition of superoxide dismutase, glutathione peroxidase in erythrocytes of rats. It has been shown that administration of quercetin under conditions of experimental diabetes reduces the severity of oxidative stress and normalizes the concentration of MDA in the red blood cells due to the stimulating effect on the activity of antioxidant enzymes (superoxide dismutase, glutathione), and improves glycemic status of the animals. These results testify that under the conditions of simulated streptozotocin diabetes red blood cells of white rats develop oxidative stress due to the activation of lipid peroxidation process and inhibition of enzyme activity of the antioxidant system. Plant flavonol quercetin can reduce oxidative stress due to the stimulating effect on the activity of antioxidant enzyme (superoxide dismutase, glutathione reductase) and inhibition of lipid peroxidation in red blood cells of animals. Introduction of quercetin against the background of streptozotocin diabetes causes a decrease in the concentration of glucose in the blood plasma and the level of glycated hemoglobin in the blood of rats in comparison with indicators peculiar to animals with streptozotocin diabetes. Results of our studies complement the existing data on the favorable effect of quercetin on erythrogenesis system cells and demonstrate the capacity of this flavonol to strengthen adaptive response in erythroid cells in response to glycemia caused by oxidative stress. Metabolic effects of quercetin indicate the appropriateness of quercetin for the correction of metabolic changes in erythroid cells, caused by diabetes mellitus type 2.

Added: 16.11.2016

Keywords: diabetic nephropathy, Еxperimental diabetes mellitus type 2, red blood cells in rats, quercetin

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