Development of pulmonary tuberculosis is accompanied by oxidative stress which is typical for the intensity of free radical processes and decrease of natural antioxidant enzymes.

Progression of pulmonary tuberculosis accompanied by significant activation of free radical processes and lipid peroxidation products release into the bloodstream that promote blood clotting, increase adhesion and aggregation of blood cells, increasing its viscosity, leading to complications and increase the severity of the disease [11]. This makes the search for new treatments for patients with pulmonary tuberculosis using physical and chemical means countervailing action.

Today we know that helium-neon laser radiation and ozone physiological solution is an effective non-medicated therapeutic agent in the treatment of many diseases. Helium-neon laser radiation has a stimulating effect on the structure of cell membranes, modulates antioxidant factors in cells that ultimately improves the reparative processes [3, 12].

One of the leading biological effects of ozone (О3) is to optimize the metabolic processes in the body due to the normalization of the balance of pro- and antioxidant systems, facilitating the synthesis of arachidonic acid and prostoglandin that reduce tissue hypoxia and adjust electrolytic balance that is essential for pulmonary tuberculosis [1, 19–22]. The starting material studies were experimental data [10, 15, 16], namely the average values of diene conjugates (DC) and TBA-positive products (TBA-PP), peroxide hemolysis of red blood cells (PHE), total oxidant activity of blood plasma (ZOA) molecules of middle mass (MMM) and indicators of activity of antioxidant enzymes superoxide dismutase (SOD) and catalase (K), protein ceruloplasmin (CP) for the actions of He-Ne laser radiation, ozone physiological solution and their combined effects.

The tested material worked on by cluster and univariate analysis of variance. Using cluster analysis incrementally analyzed the distance between the observations and clusters are created for the studied parameters and create a new cluster and steam, which was the smallest distance.

Evaluation of the combined effect of helium-neon laser (with frequency 1,0–3,0 kHz, 5,0 kHz, 100 kHz and without them), ozonized saline with ozone concentration of 8 mg/L ↑↓ and 12 mg/L ↑↓ (in increments of 2 mg/L), and combinations there of processes lipid peroxidation, antioxidant enzyme activity and the level of endogenous intoxication in patients with destructive pulmonary tuberculosis in the intensive phase of standardized chemotherapy using cluster and univariate analysis of variance.

The results suggest a positive effect of He-Ne laser (with frequency 1,0–3,0 kHz, 5,0 kHz, 100 kHz and without them), ozonized saline (with varying concentrations of О3) and their combined impact on the prooxidant-antioxidant balance in the blood of patients with pulmonary tuberculosis compared to one HT leads to reduce the intensity of lipid peroxidation and antioxidant protection enzymes increased activity in the blood of patients with pulmonary tuberculosis.

The optimum effect of ozonized saline (О3 concentration of 12 mg/L ↑, 12 mg/L ↑↓, the number of procedures 14, 18) and the combined influence of О3 (12 mg/L ↑ and 12 mg/L ↑↓, the number of procedures 14, 18) intravenous blood irradiation with frequency 100,0 kHz and 5,0 kHz on the background of chemotherapy according to the prooxidant-antioxidant balance.

Keywords: lipid peroxidation, antioxidant protection, helium-neon laser radiation, ozone physiological fluid, tuberculosis

Full text: PDF (Ukr)

1. 1. Alekhina S, Shcherbatyuk T. Ozone therapy: Clinical and Experimental Aspects. Saratov. 2004: 278.
2. 2. Afonina G, Kuyun L. Lipids, free radical, and the immune response. Kiev: NMU; 2000. 287.
3. 3. Vladimirov Y, Klebanov G, Borisenko G et al. Molecular and cellular mechanisms of action of low-intensity laser radiation. Biophysics. 2004;49(2):339-350.
4. 4. Gabrieljan N, Lipatov V. Experience of an indicator of middle molecules in the blood for the diagnosis of diseases in children nephrology. Lab. business. 1984;3:138-140.
5. 5. Derkach M, Gumetskyi R, Chaban M. The course of variational statistics. Kiev: High School; 1977. 208.
6. 6. Kolb V, Kamyshnikova V. Determination of ceruloplasmin in blood serum a modified method of Revin. Clinical Chemistry: A Handbook. Minsk. 1971. 36-37.
7. 7. Korobeinikova Z. Modification determination of lipid peroxidation products in the reaction with thiobarbituric acid. Lab. Business. 1989;7:8-10.
8. 8. Коrolyuk M, Ivanova L, Mayоrova I. Method for determination of catalase activity. Lab. Business. 1991;1:17-19.
9. 9. Ed. Horyachkovskyi A. Clinical laboratory diagnostics in biochemistry. Odessa: Ecology. 2005;275:381-382.
10. 10. Mazhak K, Otchych O, Sanagurskii D. The influence of сhеmoozonotherapy on prooxidant-antioxidant homeostasis of the blood of patients with pulmonary tuberculosis. Experimental and clinical phisiology and biochemistry. 2013;4(64):32-40.
11. 11. Menshchikova E, Zenkov N, Lakin V et al. Oxidative stress: Pathological conditionals and deseases. Novosibirsk: ARTA; 2008. 284.
12. 12. Nechypurenko N, Pashkovskaya I, Stepanova Yu et al. Mechanisms of action and biological effects. Medicine news. 2008;12:17-21.
13. 13. Eralyna S, Ismailov E, Abdrasulov R, Tekesbaev B et al. New technologies of vnutryvennoho laser irradiation of blood. Guidelines Kazakh, National University of medical behalf SD Asfendyyarova. Almata: 2014. 31.
14. 14. Chekman I, Syrovaya A, Makarov V, Makarov V et al. Ozon and Ozonotherapy: Monography. Х: Digital Print №1"; 2013. 144.
15. 15. Otchych O, Mazhak K, Sanagurskуi D. Effects of complex of ozone- and laserotherapy on prooxidant-antioxidant balance in blood of patiens with lung tuberculosis in the intensive phase chemotherapy. Studia Biologica. 2014;8(2):91-100.
16. 16. Otchych O, Mazhak K, Ivanov H et al. Membrane dependant processes in erythrocytes of blood of patients with pulmonary tuberculosis under the influence of helium-neon laser radiation. Studia Biologica. 2013;7(2):65-74.
17. 17. Holovchak N, Tarnovska A, Kotsyumbas H et al. The processes lipid peroxidation in alive organisms. Lviv: LNU I. Franko; 2012. 250.
18. 18. Stalnaya I. Method for determination of DK of unsaturated fatty acids. Modern methods in biochemistry. Мoscow: Mеdicine. 1977; 63-64.
19. 19. Bocci V. Scientific and Medical Aspects of Ozone Therapy. Archives of Medical Research. 2006;37:425-435. doi.org/10.1016/j.arcmed.2005.08.006
20. 20. Lamberto R, Mohamed N, Menеndez S et al. Ozone therapy: clinical and basic evidence of its therapeutic potential. Arch. Medical Research. 2007;3(2):231-243.
21. 21. Schwartz A, Martínez-Sánchez G.Ozone therapy and its scientific foundations. Revista espa-ola de ozonoterapia. 2012;2(1):199-232.
22. 22. Travagli V, Zanardim I, Bernini P et al. Effects of ozone blood treatment on the metabolite profile of human blood. Int. J. Toxicol. 2010;29(2):165-174.