The study was carried out on 24 white viripotent male rats of the Wistar stock, in average weight of 100 g., age 3, 5–7, 5 months. Before carrying out of the experiments, careful selection of animals was made and the condition of mucous membrane on the absence of inflammatory changes or abnormal affects was assessed.

In order to analyse oral microflora the microbiological and bacteriological studies were carried out. The material for bacterioscopic study was withdrawn from the surface of teeth on the edge of gingivae and teeth facial surface, gingivae, and from the oral cavity proper mucosal by an inoculation loop. From the material withdrawn smears were prepared and stained according to the Gram’s method. Simultaneously, the material withdrawn with the help of loop was inoculated to the growth medium, specifically to sugar broth, blood agar, egg-yolk salt agar, Endo and Sabouraud agars. The developed bacteria isolates were identified according to the complex of morpho-tinctorial and biochemical properties, involving standard differential selective media.

In microscopic examination of the smears from the surface of teeth (medial and lateral incisors – on the edge of soft tissues and facial teeth surface), microflora was of the same type in different animals. Long threadlike Gram-positive bacteria – Leptotrix were found in each field of microscope. Gram-positive microflora consisted of Gram-positive rods that in accordance with the morphological characteristics may be identified as lactobacilli. Lactobacillus is a genus of Gram-positive anaerobic nonspore-forming lactic acid rod-shaped or sometimes coccoid bacteria which can be in isolation or arranged in short chains. Coccoid Gram-positive flora consisted of staphylococci and streptococci which were mainly arranged in separate groups or aggregates. Gram-negative cocci and rods were also found. Generally, in the smears Gram-positive microflora prevailed.

Nonhemolytic and α-hemolytic streptococci, coagulase-negative staphylococci and Escherichia were isolated from the oral cavity of rats. The before-mentioned bacteria developed in large quantities from the teeth surface. The quantity of α-hemolytic streptococci was 65±7 CFU/ 0,02 ml, nonhemolytic streptococci – 45±4 CFU/ 0,02 ml, staphylococci – 10±1,5 CFU/ 0,02 ml, Escherichia – 7±0,8 CFU/ 0,02 ml.

From the mucosal oral vestibule α-hemolytic streptococci developed in the quantity of 52±4 CFU/ 0,02 ml, nonhemolytic streptococci – 25±5 CFU/ 0,02 ml, staphylococci – 8±1,5 CFU/ 0,02 ml, Escherichia – 5±0,8 CFU/ 0,02 ml.

From the oral cavity mucosal α-hemolytic streptococci developed in the quantity of 21±3,2 CFU/ 0, 02 ml, nonhemolytic streptococci – 15±1,5 CFU/ 0,02 ml, staphylococci – 9± 0,5 CFU/ 0,02 ml, Escherichia – 4±0,3 CFU/ 0,02 ml.

Therefore, saprophytic and opportunistic microflora, in particular potentially cariogenic types of α-hemolytic streptococci developed from the oral cavity of white rats.

In a microscopic examination the acidogenic bacteria – lactobacilli and Leptotrix were found. The study carried out on intact white rats in no acute distress defined the oral cavity microflora and made it possible to be used for the future comparative analysis and distress simulation in the experiment.

Ключові слова: microflora, rats, oral cavity

Повний текст: PDF (Ukr)