Among the therapeutic measures that reduce the likelihood of cerebral ischemia in stroke (CVA), a lot of interest is paid to the effect on central hemodynamics. Infusion therapy takes an important place, the choice of which in case of diseases and injuries of the brain is one of the most difficult problems in the complex conservative treatment of these patients.

The aim of this study was to characterize the effect of colloid hyperosmolar solution HAES-LX-5%, colloid solution Voluven and 0.9% NaCl solution on DNA fragmentation (apoptosis) of somatosensory cortex cells in model ischemic postreperfusion brain damage in rats.

Materials and methods. The experimental model of ischemia – reperfusion (IR) was created by clipping both internal carotid arteries for 20 min. Infusion solutions of 0.9% NaCl, HAES-LX-5% and Voluven were injected intravenously 2.5 ml/kg, 2 times/day (5 ml/kg per day). The first introduction was performed 30 min after IR and then daily every 12 hours for 4 days. The control group consisted of intact rats and animals with IR untreated. After 96 hours after IR and decapitation of animals the parts of somatosensory cortex were taken for further evaluation of DNA fragmentation in cells. The study was performed by flow cytometry.

Results. The study showed that in the control group (IR untreated) rate of DNA fragmentation in the nuclei of cells of somatosensory cortex after 96 hours after modeling 20 minutes of cerebral ischemia-reperfusion was significantly higher in 3.4 times in comparison with the group of intact rats.

Therapeutic introduction of solutions Voluven and HAES-LX-5% to rats with stroke produced a similar in direction and strength anti-apoptotic action relatively to control, manifested in the reduction of DNA fragmentation in the nuclei of cells of somatosensory cortex in the study on average by 36.3% and 47.8% (p<0.05).

Comparing the influence of solutions Voluven and HAES-LX-5% with the influence of 0.9% NaCl solution it turned out that on the 4th day of the observation infusion of HAES-LX-5% and Voluven strength of anti-apoptotic effect dominated the administration of 0.9% solution NaCl on average by 24.1 and 37.8% (both p<0.05).

When comparing the effectiveness of two test solution containing colloid base, in terms of DNA fragmentation (SUB-G0G1) nuclei somatosensory cortex in rats with IR found that treatment with a solution of HAES-LX-5% proved to be significantly better than the infusion Voluven 18.1% (13,48±0,68% to 16,45±0,82%) (p<0.05).

The obtained data is evidence that infusion therapy for CVA leads to inhibition of neuroapoptosis processes which is obviously due to the improved blood circulation in the area of cerebral ischemia by reducing dehydration and the establishment of therapeutic hemodilution.

Ключові слова: ischemia-reperfusion injury of the brain, normal saline, HAES-LX-5%, Voluven, apoptosis

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