Connective tissue proteins collagen and elastin in 13% consist of oxyproline. It is generally accepted that the content oxyproline in connective tissue significantly correlated with indices of collagen. Healing intestinal wounds depends on the strength of the collagen mesh, which is a matrix of connective tissue that forms a scar. The definition of oxyproline in the small intestine in the region of the anastomosis after resection makes it possible to evaluate the effectiveness of wound reparative processes. Cronin K. et al (1968) reported the transient decrease hydroxyproline of colonic anastomosis after operation.

Similar results were obtained with enteric anastomosis. The aim of our research was to study the dynamics of the oxyproline of the area of anastomosis end-to-end after resection of the small intestine in experimental conditions.

The experiment was performed on sexually mature males rabbits in the operating conditions of the vivarium and laboratory of Toxicology and Analytical Chemistry. Surgical intervention was carried out under the European Convention for the Protection of Vertebrate Animals used for experimental and other scientific purposes (Strasbourg, 1986), the Law of Ukraine “On protection of animals from cruelty” and were approved due to the local Ethics Committee.

In operating room of vivarium, subject to the rules of aseptic and antiseptic, used medium median laparotomnoy access. Intestinal sewed end-to-end single node sero-muscular sutures using microsurgical instruments and atraumatic suture material prolen 6,0. Laparotomy wound sewn seams single node through all layers. Definition of the oxyproline in segment of the small intestine with anastomosis on the third, seventh, fourteenth day after the operation was developed. A part of intestine was crushed in a mortar. 0,1–0,4 g of tissue were taken and homogenized with 3 ml of distilled water in homogenizer HG-15A-Set. To blenders 5 ml of concentrated hydrochloric acid were added, closed tightly in a bottle and heated to 120°C for 16 hours. After 16 hours the contents of the vial were transferred to a porcelain cup and evaporated liquid from the test material in water bath. The dry residue was dissolved in distilled water and the total volume of the solution was adjusted to 10 ml and centrifuged in a Engelsdorf-Leipzig Janetzki centrifuge for 10 min. at a speed of 5000 rev/ min. 4 ml of the supernatant was brought into the test tube, added 2 ml of 1% solution of chloramine B, stirred and left for 20 minutes. After that added to 2 mL of 3,15 M solution of perchloric acid, left for 5 minutes, adding 2 ml Ehrlich reagent and heated keeping the temperature of 60°C for 20 minutes. We used spectrophotometry for determination of hydroxyproline on СФ-46 at a wavelength of 480 nm.

The concentration of hydroxyproline intact wall of the small intestine ranged from 33,02 to 38,24 mg/100 g. On the third day after the operation hydroxyproline compounds decreased slightly from 29,02 to 36,23 mg/100 g. Over the next four days the concentration of hydroxyproline increased, and on the seventh day was determined in the range from 118,23 to 152,38 mg/100 g. On the fourteenth day fixed concentration of hydroxyproline of anastomosis area from 144,63 to 175,22 mg/100g.

The technique of determining oxyproline tissues is effective and can be used to study intestinal wound healing process. Within three days after the operation, the concentration of oxyproline of intestinal anastomosis area slightly reduced compared with the intact intestine. During the next two weeks oxyproline level rose, this is probably associated with the formation of a scar.

Keywords: connective tissue, hydroxyproline, intestinal wound

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