The article presaents the results of the study of changes in the amino acids saliva content in patients with cholecystitis, chronic pancreatitis and reactive hepatitis. Twelve separate fractions of free amino acids are identified by paper chromatography in saliva samples collected from 22 patients with cholecystitis, chronic pancreatitis, hepatitis, and from 18 healthy volunteers. It was found that in patients with cholecystitis, chronic pancreatitis and hepatitis reduced saliva concentration of histidine, taurine, arginine, serine, glycine and aspartic acid, but increases the concentration of glutamic acid, threonine, alanine, methionine, leucine and tryptophan. That is, patients with cholecystitis, chronic pancreatitis and hepatitis experiencing significant changes in the spectrum of free amino acids saliva such that indicates a change in the amino acid and protein metabolism. Significant differences in the concentration of free amino acids in the saliva of healthy men and women were revealed. In the women saliva of the control groupcontained much less cysteine and cystine (40% decrease, p<0,01), histidine and taurine (75% decrease, p<0,001), aspartate and glycine (53% decrease, p<0,001 ), isoleucine and phenylalanine (77,1% decrease, p<0,001) than in the saliva of male control group. It was found that ornithine and lysine concentration in the saliva of women in the control groupis 43,75% (p<0,001) higher than in men. It was shown that the content of asparagine and histamine in saliva was smaller in men than in women by 34,8% (p<0,001). Saliva glutamic acid and threonine concentration are less by 30.4% (p<0,001), proline and hydroxyproline – by 32,1% (p<0,001), alanine and methionine – by 48,6% (p<0,001), valine and tyrosine – by 41,4% (p<0,001), leucine and tryptophan – by 34% (p<0,001) in men compared to women. In female patients, compared with healthy women, there is a reduction of saliva ornithine and lysine by 37,5% (p<0,001), histidine and taurine – 50% (p<0,001), aspartate and glycine – 46,8%. It was shown that a significant change in the concentration of free amino acids occurs in the saliva of men patients with cholecystitis, chronic pancreatitis and reactive hepatitis (in their saliva decreases the content of histidine and taurine – by 68,1% (p<0,001), aspartate and glycine – by 49,1% (p<0,01), isoleucine and phenylalanine – 41,5 % (p<0,05) and increase concentration of asparagine and histamine – by 93,3% (p<0,05), glutamate and threonine at 2,87 times (p<0,001), alanine and methionine – to 4-fold (p<0,001), valine and tyrosine 2,4 (p<0,01), leucine and tryptophan to 3-fold (p<0,01)). Thus, the content of histidine, aspartate, taurine and glycine decreases in the saliva of both male and female groups. It is shown that significant changes in the concentration of free amino acids occur in the saliva of male patients with cholecystitis, chronic pancreatitis and reactive hepatitis. Investigation of the amino acid composition of saliva can provide valuable additional information to developthe most effective treatment strategy for the mentioned complex pathologies.

Ключові слова: amino acids, saliva, cholecystitis, reactive hepatitis, pancreatitis

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