Abstract. Cyanoacrylate glue is extensively applied across multiple fields of medicine owing to its capacity to provide hermetic tissue adhesion, its pronounced hemostatic and bacteriostatic effects, biocompatibility, and gradual biodegradation within the body. However, its application may also exert adverse effects on the morphological characteristics of tissues.

The aim of the work was to investigate the morphological changes in simple renal cysts along the bonding line following the application of cyanoacrylate glue in an in vitro experiment.

Materials and methods. A fragment of the wall of a simple renal cyst removed from the human body by surgical treatment (laparoscopic excision) was glued using cyanoacrylate glue. Ultra-thin sections were prepared on UMTP-6 ultramicrotome with a diamond knife, contrasted for 15 min with a 2 % uranyl acetate solution and additionally with lead citrate by Reynolds [1]. The sections were viewed and photographed on a TEM-100 transmission electron microscope.

The results. It was established that the application of cyanoacrylate glue for sealing the walls of a kidney cyst (in vitro) results in morphological alterations of the tissues, particularly affecting the structure of the epithelium and connective tissue.

Conclusions. The study demonstrated that the application of cyanoacrylate glue for sealing the walls of a kidney cyst in vitro induces structural alterations in the epithelium, hemocapillaries, and connective tissue.

Ключові слова: сyanoacrylate glue, kidney cyst, morphology

Повний текст: PDF (Ukr)

1. Reynolds ES. The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J. Cell Biol. 1963; 17: 208-12.doi.org/10.1083/jcb.17.1.208
2. Ayyıldız SN, Ayyıldız A. Cyanoacrylic tissue glues: Biochemical properties and their usage in urology. Turk. J. Urol. 2017; 43: 14-24.doi.org/10.5152/tud.2017.09465
3. Amiel GE., Sukhotnik I, Kawar B, Siplovich L. Use of N-butyl-2-cyanoacrylate in elective surgical incisions-longterm outcomes. J Am Coll Surg. 1999; 189: 21-5.doi.org/10.1016/S1072-7515(99)00068-X
4. De Concilio B, Vedovo F, Mir MC, Silvestri T, Casarin A, Celia A. Gelatin sponge (Spongostan®) and N-butyl-2-cyanoacrylate: Utility on percutaneous treatment of persistent urinary leakage after partial nephrectomy. Case report and review of the literature. Arch Ital Urol Androl. 2020; 92: 200-2. doi.org/10.4081/aiua.2020.3.200
5. Doppalapudi SK, Wajswol E, Shukla PA, Kolber MK, Singh MK, Kumar A. et al. Endovascular Therapy for Vasculogenic Erectile Dysfunction: A Systematic Review and Meta-Analysis of Arterial and Venous Therapies. J Vasc Interv Radiol. 2019; 30: 1251-8.e2.doi.org/10.1016/j.jvir.2019.01.024
6. Savoliuk SI, Shepetko-Dombrovskyi OH, Shepetko-Dombrovskyi HM. Biological sealant "Sulfakrylat" in laparoscopic surgery of perforated duodenal ulcer. Hospital Surgery. Journal Named by L.Ya. Kovalchuk, no. 4. 2017: 100-2. https://doi.org/10.11603/2414-4533.2016.4.7200. doi.org/10.11603/2414-4533.2016.4.7200