Abstract. Currently, the role of the intestinal microbiota in the development of obesity is debated due to its potential to improve energy metabolism, therefore, studies of the pathogenesis of metabolic-associated fatty liver disease under the conditions of probiotic use remain relevant.

The aim of the study is to determine morphological changes in liver tissue and the level of mRNA expression of the Ptgs2 and Tgfb1 genes in rat hepatocytes under conditions of glutamate-induced steatohepatosis and correction with the concentrated multiprobiotic «Symbiter acidophilus».

Methods. The direction included the study of morphological changes in liver tissue and determination of the mRNA expression level of Ptgs2, Tgfb1 genes in rat hepatocytes under conditions of neonatal administration of monosodium glutamate (4 mg/g, dissolved in water for injection in a volume of 8 μl/g, subcutaneously) and against the background of periodic use of the multiprobiotic «Symbiter acidophilus» concentrated (0.14 ml/kg in a volume of 0.25 ml/100 g, orally).

Results. In 4-month-old rats, which were administered monosodium glutamate in the neonatal period, visceral obesity without hyperphagia was recorded, which was characterized by the development of steatohepatosis. Morphological changes in the liver tissue of rats with glutamate-induced obesity indicate the development of severe steatosis, lobular inflammation, and ballooning dystrophy of hepatocytes. In rats with glutamate-induced steatohepatosis, mRNA expression levels of Ptgs2 and Tgfb1 genes in hepatocytes increase, which indicates the development of an inflammatory process and fibrosis in liver tissue.

Conclusions. Periodic administration of the concentrated multiprobiotic «Symbiter acidophilus» to rats with glutamate-induced obesity restores the morphological state of the liver, namely: eliminates the appearance of balloon dystrophy, significantly reduces lobular inflammation and the severity of steatosis. In rats with steatohepatosis, which were periodically administered a multiprobiotic, the level of mRNA expression of the Ptgs2 and Tgfb1 genes in hepatocytes was completely restored to the control level.

Keywords: monosodium glutamate, obesity, steatohepatosis, mRNA, Ptgs2 and Tgfb1 expression, multiprobiotic «Symbiter acidophilic» concentrated

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