Abstract. The increasing number of obese people and the significant increase in injured patients make it urgent to study the process of re-epithelialization and the search for new dermatotropic agents.

Purpose. To investigate the features of healing of full-thickness excised planar wounds in rats of different sexes with glutamate-induced obesity (GIO).

Materials and methods. The study was conducted on 64 white non-linear rats aged 4 months, 32 males and 32 females, which were separately divided into 4 groups. Rats of groups I and II were intact. In rats of groups III and IV, both males and females, obesity was simulated by administering monosodium glutamate (MSG) in the early neonatal period. In 4-month-old rats of all groups, full-thickness excised planar wounds were simulated. In the dynamics of healing, the area of the wounds was measured.

Results. In 4-month-old male and female rats, which were administered MSG in the early neonatal period, obesity was recorded, including visceral, which was more pronounced in males. In males with GIO, the duration of healing and the area of lesions throughout the experiment were greater compared to controls. In females with GIO, the healing time was the same as in the control, but the lesion area during the experiment was larger compared to the control. In males and females with GIO, daily treatment of wounds with carbopol gel with melanin accelerated wound healing and significantly reduced the area of lesions during the experiment.

Conclusions. In rats of both sexes with GIO, the healing process of fullthickness excised planar wounds was impaired. Carbopol gel with melanin had a pronounced dermatotropic effect.

Ключові слова: glutamate-induced obesity, full-thickness excised planar wounds, carbopol gel with melanin

Повний текст: PDF (Ukr)

1. Wang Z, Wang Y, Bradbury N, Bravo CG, Schnabi B, Di Nardo A. Skin wound closure delay in metabolic syndrome correlates with SCF deficiency in kerationocytes. Sci Rep. 2020 Dec;10(1):21732.doi.org/10.1038/s41598-020-78244-y
2. Bashir S, Sharma Y, Khan F. Macrophage Polarixation: the Link between Inflammation and Related Diseases. Inflamm Res. 2016;65(1):1-11.doi.org/10.1007/s00011-015-0874-1
3. Strollo F, Gentile S, Pipicelli AMV, Mambro A, Monici M, Magni P. Space Flight-Promoted Insulin Resistance as a Possible Disruptor of Wound Healing. Front Bioeng Biotechnol. 2022 May;10:868999.doi.org/10.3389/fbioe.2022.868999
4. Solano F. Photoprotection and Skin Pigmenation: Melanin-Related Molecules and Some Other New Agents Obtained from Natural Sources. Molecules. 2020 Mar;25(7):1537.doi.org/10.3390/molecules25071537
5. Agrawal M. Natural polyphenols based new therapeutic avenues for advanced biomedical applications. Drug metabolism reviews. 2015;47(4):1-11.doi.org/10.3109/03602532.2015.1102933
6. Hosnuter M. The protective effects of epigallocatechin gallate against distant organ damage after severe skin burns - experimental study using a rat model of thermal trauma. Advances in Clinical and Experimental Medicine. 2015;24(3):409-417.doi.org/10.17219/acem/43761
7. Taburets OV, Morgaienko OO, Kondratyuk TO, Beregova TV, Ostapchenko LI. The Effect of «Melanin-Gel» on the Wound Healing. Research Journal of Pharmaceutical, Biological and Chemical Sciences (RJPBCS). 2016;7(3): 2031-2038.
8. Taburets O. V., Grinchenko O. O., Dvorschenko K. O., Vereschaka V. V., Ostapchenko L. I. Influence of the melanin on the state of prooxidant-antioxidant homeostasis in blood serum at the rats with the full-thickness skin wound. Bulletin of problems in biology and medicine. 2017;1:191-196.
9. Sanabria ER, Pereira MF, Dolnikoff MS, Andrade IS, Ferreira AT, Cavalheiro EA, Fernandes MJ. Deficit in hippocampal long-term potentiation in monosodium glutamate-treated rats. Brain Res Bull. 2002;59:47-51.doi.org/10.1016/S0361-9230(02)00837-7
10. Bernardis LL, Patterson BD. Correlation between 'Lee index' and carcass fat content in weanling and adult female rats with hypothalamic lesions. J Endocrinol. 1968;40:527-528.doi.org/10.1677/joe.0.0400527
11. Garg SS, Kushwaha, Dubey R, Gupta J. Association between obesity, inflammation and insulin resistance: Insights into signaling pathways and therapeutic interventions. Diabetes Res Clin Pract. 2023 Jun; 200:110691.doi.org/10.1016/j.diabres.2023.110691
12. Zulkefli N, Zahari CNMC, Sayuti NH, Kamarudin AA, Saad N, Hamezah HS, et al. Flavonoids as Potential Wound-Healing Molecules: Emphasis on Pathways Perspective. Int J Mol Sci. 2023 Feb 27;24(5):4607.doi.org/10.3390/ijms24054607
13. Chanu NR, Gogoi P, Barbhuiya PA, Dutta PP, Pathak MP, Sen S. Naural Flavonoids as Potential Therapeutics in the Management of Diabetic Wound: A Review. Curr Top Med Chem.2023;23(8):690-710.doi.org/10.2174/1568026623666230419102140
14. Carvalho MTB, Araújo-Filho HG, Barreto AS, Quintans-Júnior LJ, Quintans JSS, Barreto RSS. Wound Healing properties of flavonoids: A systematic review highlighting the mechanisms of action. Phytomedicine. 2021 Sep; 90:153636.doi.org/10.1016/j.phymed.2021.153636