Abstract. Lead and fluoride enter the human body individually and simultaneously through water, food, and air, disrupting metabolic processes in liver tissue. The sequential application of a bioprotective complex that reduce the negative effects of lead and fluoride has been insufficiently studied.

The aim of the study is to investigate the changes over time in the activity of alanine aminotransferase and aspartate aminotransferase in the blood serum of laboratory animals under prolonged separate and simultaneous exposure to lead and fluoride, without application of bioprotective agents and during the sequential addition of pectin, pectin with calcium, and a complex of pectin, calcium, and antioxidants to the standard animal diet.

Materials and Methods. Four clinical trial series were conducted on white rats, each following an orthogonal design 2 2 . Aqueous solutions of Pb(NO 3 ) 2 and NaF were administered orally separately and simultaneously over 30 days: in the first series without bioprotective agents, in the second with pectin, in the third with pectin and calcium, and in the fourth with a complex of pectin, calcium, and antioxidants – C, E, and β-carotene vitamins, and selenium. The activity of alanine and aspartate aminotransferases was measured in blood serum on days 3, 15, and 30 in the first and second series of experiments, and on day 30 in the third and fourth series.

Results. Separate and simultaneous oral administration of lead and fluoride to white rats leads to increased activity of alanine and aspartate aminotransferases in blood serum. The maximum changes were observed at the end of the experiment. The protective role of pectin alone under long-term fluoride expos ure and simultaneous exposure to lead and fluoride was not sufficiently effective. Concurrent intake of calcium and pectin significantly reduced the increase in transaminase activity, indicating an improvement in the metabolic and functional state of the liver. The application of the bioprotective complex restored the aminotransferase activity in blood serum.

Conclusions. The complex of pectin with calcium and antioxidants can be considered an optimal means of correcting aminotransferase activity in blood serum under prolonged separate and simultaneous exposure to lead and fluoride.

Article received: 12.06.2024

Keywords: lead, fluoride, blood serum, aminotransferase, pectin, calcium, antioxidants, separate and simultaneous exposure

Full text: PDF (Ukr)

1. Environmental Health Criteria. 165. Inorganic Lead. Geneva : WHO, 1995; 251 p.
2. Environmental health criteria. 227. Fluorides. Geneva : WHO, 2002; 268 p.
3. Trakhtenberg IM, Dmytrukha NM, Lugovskyi SP, Chekman IS, Kuprii VO, Doroshenko AM. Lead is a dangerous pollutant. The old and new problem. Ukrainian Journal of Modern Toxicological Aspects. 2015;3:14-24.
4. Lubojanski A, Piesiak-Panczyszyn D, Zakrzewski W, Dobrzynski W, Szymonowicz M, Rybak Z, et al. The Safety of Fluoride Compounds and Their Effect on the Human Body-A Narrative Review. Materials (Basel). 2023 Jan 31;16(3):1242.doi.org/10.3390/ma16031242
5. Guth S, Hüser S, Roth A, Degen G, Diel P, Edlund K, et al. Toxicity of fluoride: critical evaluation of evidence for human developmental neurotoxicity in epidemiological studies, animal experiments and in vitro analyses. Arch Toxicol. 2020 May;94(5):1375-1415.doi.org/10.1007/s00204-020-02725-2
6. Barbier O, Arreola-Mendoza L, Del Razo LM. Molecular mechanisms of fluoride toxicity. Chem Biol Interact. 2010 Nov 5;188(2):319-33.doi.org/10.1016/j.cbi.2010.07.011
7. Grzehotskyi MR. Conceptual model of preventive medicine from the standpoint of human physiology (literature review and own research). Journal of the Academy of Medical Sciences of Ukraine.2003;9(2):312-324.
8. Bioprofilaktyka rozvytku ekozalezhnoyi patolohiyi u krytychnykh verstv naselennya industrial'nykh mist: metodychni rekomendatsiyi.К., 2010; 20 c.
9. Antomonov МY, Korobeinikov HV, Khmelnytska ІV, Kharkovliuk-Balakina NV. Mathematical methods of processing and modeling the results of experimental studies. Kyiv: Olimpiiska literatura.2021;216 с.
10. Bagmut I, Kolisnyk I, Titkova A, Babiy L, Filipchnko S. The antioxidant system enzymes' activity in rats' brain, toxicated with sodium fluoride in subtoxic doses. Arch Balkan Med Union.2018;53(4):506-11.doi.org/10.31688/ABMU.2018.53.4.03
11. Reznikov OH, Polumbryk ОМ, Balion YH, Polumbryk MО. Pro- and antioxidant systems and pathological processes in humans. Visn. Nac. Akad. Nauk Ukr. 2014;10:7-29.
12. Федоренко Ю. В. Динаміка змін активності амінотрансфераз у сироватці крові білих щурів за умов дії свинцю на фоні застосування біопротекторів // Зб. матеріалів VI науково-практичної internet-конференції з міжнародною участю «Механізми розвитку патологічних процесів і хвороб та їх фармакологічна корекція» 16 листопада 2023 р. Харків - Україна:481-2.https://pat.nuph.edu.ua/wp-content/uploads/2023/11/zbirnyk-materialiv-konferentsii-16.11.2023.31.03.pdf
13. Fedorenko YuV. Metabolic and functional state of the liver at the combined effect lead and fluorine on the background of bioprotectors application One Health and Nutritions